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KMID : 0364819870250020122
Korean Journal of Microbiology
1987 Volume.25 No. 2 p.122 ~ p.128
Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons.





Abstract
1
Several bacterial isolates capable of degrading 4-chlorobiphenyl or 2,4,5-trichlorophenoxyaeetic UN were Isolated from industrial wastes by the agar plate method and studied for their biodegradabiiities of the hydrocarbons and some biochemical characteristics. The Isolates DJ-12,1)1-26 and TP-1 were Identified as Pseudomoner spp. and they could not degrade 2,4-dkhloropbeuoxyacetlc acid. The absorption spectra for 4-chloroblphnyl and 2,4,5-triehloropheeoxyacetic add showed the peaks at 253 and 292 am, respectively. Biodegradability of the isolates was determined by decease of the absorbance for the teat hydrocarbons with a UV-sauniag spectrophotometer. The pbuauids of the isolates were studied to examine whether or not the hydrocarbon-degradi g genes exist in the plasaids. Antibiotics resistance was also examined to search out a proper marker for the isolates in further experiments, such as caring test and genetic recombination.
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